共计 1099 个字符，预计需要花费 3 分钟才能阅读完成。
There are no universal rules for naming plasmids but here are some good guidelines to follow in order to ensure that people can quickly and easily identify what your plasmid contains and other important information.
目前命名质粒的方法并没有国际统一的策略，本站现在公布一下几个原则便于分类和快速的辨别你构建的质粒。
第一步 Step 1: Backbone Name
Include the empty backbone name in your plasmid name. This simple piece of information can often convey
many important details. Once you know the backbone a plasmid is based on, you can usually derive: a) the
bacterial antibiotic resistance, b) the promoter that drives the insert, and c) any other selection markers (for
use in other cell types, e.g. eukaryotic cells).
pBACKBONE-XXXXX
第二步 Step 2: Insert Name
Include information about the insert in your plasmid name. This is often a 3-6 letter representation of the
gene (or DNA sequence).
pBACKBONE-hGene
第三步 Step 3: Add Your Tags
Add any tags or fusions that are on your insert. Typically you would list any tag or fusion protein in the order
they appear in the plasmid and their relative position to the insert. For example, if you have a Flag tag on the
N-terminal of your insert, you would list it first.
pBACKBONE-Flag-hGene
If there was also an EGFP fused to the C-terminal of your insert you would list it after the insert.
pBACKBONE-Flag-hGene-EGFP
