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Day1:
Seed cells
onto a 96-well plate(96 wells plate, 1×104 cells per well)
Day2:
Spin down
cells(1000rpm, 5min), Aspirate all medium with a Pasteur pipet(with a 10µl“tip”
in the front)
↓
Rinse with
new medium(100 µl/well) by pipette
↓
discard
medium by suction
↓
add 100 µl
(medium + MTT) sol. to each well
(*preparing
:【10ml
medium + 1ml MTT】/plate)
5 mg MTT in 1 ml PBS
↓
back to
incubator no more than 4 hr.
↓
spin down
cells(1000rpm, 5min), discard sup.
↓
add 100 µl (isopropanol( 异丙醇) + HCl)
670µl 12N
HCl in 200ml isopropanol(final-0.04N HCl)
or add 100µl
DMSO
↓
stand for
2hr.
↓
MTT 测量波长 570nm。measure O.D.
value(570nm)
